Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyridoxal | 0.1 mM, substrate inhibition | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
ATP | pH 7, 37°C | Trypanosoma brucei | |
0.022 | - |
pyridoxal | pH 7, 37°C | Trypanosoma brucei | |
0.038 | - |
pyridoxal | pH 7, 37°C, recombinant enzyme | Trypanosoma brucei | |
0.127 | - |
ATP | pH 7, 37°C, recombinant enzyme | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, enzyme exists as dimer in solution, SDS-PAGE | Trypanosoma brucei |
73000 | - |
gel filtration | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.52 | - |
- |
Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | - |
Trypanosoma brucei | ADP + pyridoxal 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, enzyme exists as dimer in solution, SDS-PAGE | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
pyridoxal | pH 7, 37°C, recombinant enzyme | Trypanosoma brucei |