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Literature summary for 2.7.1.35 extracted from

  • Safo, M.K.; Musayev, F.N.; di Salvo, M.L.; Hunt, S.; Claude, J.B.; Schirch, V.
    Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases (2006), J. Bacteriol., 188, 4542-4552.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 20 mM potassium phosphate, pH 7.5, 5 mM beta-mercaptoethanol, 0.2 mM EDTA, and 21% PEG 4K, 100 mM Tris-HCl, pH 8.5, 200 mM Na acetate, 40 mM MgSO4, and 10% glycerol Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ in the absence of any potassium ion, the activity is less than 5% of the maximum activity Escherichia coli
Mg2+ required Escherichia coli
additional information the presence of 100 mM NaCl does not alter the potassium activation profile Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P40191
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxine
-
 Escherichia coli ADP + pyridoxine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ePL kinase 1
-
 Escherichia coli
PdxK
-
 Escherichia coli
pyridoxal kinase 1
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli