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Literature summary for 2.7.1.35 extracted from

  • Gandhi, A.K.; Ghatge, M.S.; Musayev, F.N.; Sease, A.; Aboagye, S.O.; di Salvo, M.L.; Schirch, V.; Safo, M.K.
    Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase (2009), Biochem. Biophys. Res. Commun., 381, 12-15.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tag, expressed in Escherichia coli Rosetta (lambdaDE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
D235A active site residue Homo sapiens
D235N active site residue Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
pyridoxal wild-type protein Homo sapiens
0.058
-
pyridoxal D235N mutant protein Homo sapiens
0.17
-
ATP D235A mutant protein Homo sapiens
0.17
-
pyridoxal D235A mutant protein Homo sapiens
0.17
-
ATP ATP in form of MgATP Homo sapiens
0.18
-
ATP D235N mutant protein Homo sapiens
0.18
-
ATP ATP in form of MgATP Homo sapiens
0.19
-
ATP wild-type protein Homo sapiens
0.19
-
ATP ATP in form of MgATP Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O00764
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion adsorption chromatography (Ni2+) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
Homo sapiens ADP + pyridoxal 5'-phosphate
-
?
pyridoxal + ATP
-
Homo sapiens pyridoxal 5'-phosphate + ADP
-
?

Synonyms

Synonyms Comment Organism
pyridoxal kinase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
pyridoxal D235A mutant protein Homo sapiens
0.23
-
pyridoxal D235N mutant protein Homo sapiens
0.48
-
pyridoxal wild-type protein Homo sapiens