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Literature summary for 2.7.1.36 extracted from
- Mevalonic kinase in rabbit liver (1961), Biochim. Biophys. Acta, 47, 327-335.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | or to a lesser extent other SH-compounds required | Oryctolagus cuniculus |  |
| phosphate | required for activity | Oryctolagus cuniculus | |
General Stability
| General Stability | Organism |
|---|---|
| almost complete loss of activity after dialysis against 1000 volumes of water, 2-mercaptoethanol, EDTA or glutathione | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | activity can be almost completely restored by incubation with an excess of cysteine | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
DL-mevalonate | pH 7.0, 35°C | Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Oryctolagus cuniculus | |
| Mg2+ | Mn2+ or Mg2+ required | Oryctolagus cuniculus | |
| Mg2+ | most active divalent cation | Oryctolagus cuniculus | |
| Mn2+ | divalent cation required for activity | Oryctolagus cuniculus | |
| Mn2+ | maximal activation at approx. 0.0013 mM | Oryctolagus cuniculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, protamine sulfate, ammonium sulfate, DEAE-cellulose | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% loss of activity after 1 month | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + mevalonate | ATP is the most effective phosphate donor | Oryctolagus cuniculus | ADP + phosphomevalonate | - |
? | |
| ATP + mevalonate | no activity with ADP, GTP and UTP | Oryctolagus cuniculus | ADP + phosphomevalonate | - |
? | |
| ITP + mevalonate | 36% of activity with ATP | Oryctolagus cuniculus | IDP + phosphomevalonate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 37 | assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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