Literature summary for 2.7.1.36 extracted from

Tanaka, R.D.; Schafer, B.L.; Lee, L.Y.; Freudenberger, J.S.; Mosley, S.T.
Purification and regulation of mevalonate kinase from rat liver (1990), J. Biol. Chem., 265, 2391-2398.

Search for more literature for 2.7.1.36

General Stability

General Stability	Organism
freezing of pure enzyme causes irreversible loss of activity	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
farnesyl diphosphate	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.27	-	(RS)-mevalonate	pH 7.0, 25°C	Rattus norvegicus
1.75	-	ATP	pH 7.0, 25°C	Rattus norvegicus
1.75	-	ATP	ATP in form of MgATP2-	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39900	-	2 * 39900, SDS-PAGE	Rattus norvegicus
86000	-	gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + mevalonate	Rattus norvegicus	enzyme may play a key role in regulating cholesterol biosynthesis	ADP + phosphomevalonate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, Blue-Sepharose, ammonium sulfate, Sephadex G-150, ATP-agarose	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
31.67	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (R)-mevalonate	-	Rattus norvegicus	ADP + (R)-5-phosphomevalonate	-	?
ATP + mevalonate	-	Rattus norvegicus	ADP + phosphomevalonate	-	?
ATP + mevalonate	enzyme may play a key role in regulating cholesterol biosynthesis	Rattus norvegicus	ADP + phosphomevalonate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 39900, SDS-PAGE	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0025	-	farnesyl diphosphate	pH 7.0, 25°C	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	isoelectric focusing	-	6.2

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Release 2023.1 (January 2023)