Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
expressed in Escherichia coli BL21(DE3) | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phosphomevalonate | product inhibition, non-competitive with ATP and mevalonate | Staphylococcus aureus | |
farnesyl diphosphate | feedback inhibition, competitive with ATP | Homo sapiens | |
farnesyl diphosphate | feedback inhibition, competitive with ATP | Rattus norvegicus | |
farnesyl diphosphate | feedback inhibition, competitive with ATP | Staphylococcus aureus | |
farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Homo sapiens | |
farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Rattus norvegicus | |
farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Staphylococcus aureus | |
mevalonate | substrate inhibition at millimolar concentrations, 50% inhibition at 2.88 mM | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
R,S-mevalonate | pH 7.5, 30°C | Staphylococcus aureus | |
0.339 | - |
ATP | pH 7.5, 30°C | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Staphylococcus aureus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus | |
Mn2+ | 25% of the activity with Mg2+ | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, SDS-PAGE and gel filtration | Staphylococcus aureus |
34500 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Rattus norvegicus | involved in cholesterol synthesis | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Homo sapiens | involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Staphylococcus aureus | involved in isopentenyl diphosphate synthesis | ADP + (R)-5-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.4 | - |
purified enzyme, pH 7.5, 30°C | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Staphylococcus aureus | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Homo sapiens | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | involved in cholesterol synthesis | Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome | Homo sapiens | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | involved in isopentenyl diphosphate synthesis | Staphylococcus aureus | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + mevalonate | - |
Staphylococcus aureus | ADP + phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE and gel filtration | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000029 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Homo sapiens | |
0.000035 | - |
farnesyl diphosphate | pH 7.5, 30°C | Homo sapiens | |
0.00035 | - |
farnesyl diphosphate | pH 7.5, 30°C | Rattus norvegicus | |
0.00047 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Rattus norvegicus | |
0.045 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Staphylococcus aureus | |
0.046 | - |
farnesyl diphosphate | pH 7.5, 30°C | Staphylococcus aureus |