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Literature summary for 2.7.1.39 extracted from

  • Thoen, A.; Rognes, S.E.; Aarnes, H.
    Biosynthesis of threonine from homoserine in pea seedlings: I. Homoserine kinase (1978), Plant Sci. Lett., 13, 103-112.
No PubMed abstract available
Search for more literature for 2.7.1.39

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 100 mM NH4Cl results in 35% of the activation compared to 100 mM K+ Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
6-hydroxy-DL-Lys 5 mM, 92% inhibition Pisum sativum
L-Arg 10 mM, 13% inhibition Pisum sativum
L-Cys 10 mM, 25% inhibition Pisum sativum
L-Ile 7.5 mM, 90% inhibition Pisum sativum
L-Leu 10 mM, 20% inhibition Pisum sativum
L-Lys 10 mM, 35% inhibition Pisum sativum
L-Met 10 mM, 17% inhibition Pisum sativum
L-Orn 10 mM, 64% inhibition Pisum sativum
L-Ser 10 mM, 10% inhibition Pisum sativum
L-Thr 10 mM, 18% inhibition Pisum sativum
L-Val 16 mM, 86% inhibition Pisum sativum
O-Phospho-DL-homoserine 10 mM, 91% inhibition Pisum sativum
S-adenosyl-L-methionine 10 mM, 92% inhibition Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 ATP pH 8.5 Pisum sativum
6.7
-
 L-homoserine pH 8.5 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ ineffective Pisum sativum
K+ absolute requirement for monovalent cation. Km: 21 mM Pisum sativum
Mg2+ absolute requirement for divalent cation, Mg2+ or Mn2+ Pisum sativum
Mn2+ absolute requirement for divalent cation, Mg2+ or Mn2+ Pisum sativum
Zn2+ ineffective Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 2 peaks of MW 120000 Da and 240000 Da are detected by gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-homoserine Pisum sativum the enzyme catalyzes a reaction common to synthesis of Thr, Ile and Met in plants ADP + O-phospho-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 L. cv. Pillert Fenomen
-

Purification (Commentary)

Purification (Comment) Organism
partial Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Pisum sativum
-
shoot green Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-25°C, purified enzyme is stable for at least 1 month Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homoserine
-
 Pisum sativum ADP + O-phospho-L-homoserine
-
 ?
ATP + L-homoserine the enzyme catalyzes a reaction common to synthesis of Thr, Ile and Met in plants Pisum sativum ADP + O-phospho-L-homoserine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 homoserine and MgATP2- do not protect against heat inactivation Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
8.1 9 the enzyme shows good activity in Tris-HCl buffer from pH 8.1 to pH 9.0 Pisum sativum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.9
-
 L-Val pH 8.5 Pisum sativum
0.9
-
 L-Ile pH 8.5 Pisum sativum
4.3
-
 L-Orn pH 8.5 Pisum sativum