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Literature summary for 2.7.1.4 extracted from

  • Copeland, L.; Harrison, D.D.; Turner, J.F.
    Fructokinase (fraction III) of pea seeds (1978), Plant Physiol., 62, 291-294.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
freezing and thawing after elution from the first DEAE-cellulose column results in 30% loss of activity Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Pisum sativum
D-fructose
-
Pisum sativum
D-fructose 6-phosphate 25% inhibition at 6 mM Pisum sativum
D-glucose no inhibition Pisum sativum
D-glucose 6-phosphate 25% inhibition at 6 mM Pisum sativum
Mg2+ inhibition by excess of either MgCl2 or ATP Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pisum sativum
0.06
-
ATP 30°C, pH 8 Pisum sativum
0.06
-
D-fructose 30°C, pH 8 Pisum sativum
0.06
-
ATP ATP in form of MgATP2- Pisum sativum
0.14
-
D-glucose 30°C, pH 8 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ slight stimulation Pisum sativum
K+ stimulates Pisum sativum
Li+ slight stimulation Pisum sativum
Mg2+ required Pisum sativum
Mg2+ Km: 0.06 mM for MgATP2- Pisum sativum
Mg2+ maximal activity when ATP and MgCl2 are present at approximately equal concentrations, inhibition by excess of either MgCl2 or ATP Pisum sativum
Na+ slight stimulation Pisum sativum
NH4+ stimulates Pisum sativum
Rb+ stimulates Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Pisum sativum
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
L. var. Progress
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme fructokinase III Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.094
-
-
Pisum sativum

Storage Stability

Storage Stability Organism
-18°C, stable for at least 2 months Pisum sativum
4°C, 50% loss of activity after 24 h Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Pisum sativum ADP + D-fructose 6-phosphate
-
?
ATP + D-fructose specific for fructose Pisum sativum ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose
-
Pisum sativum ADP + D-glucose 6-phosphate
-
?
additional information
-
Pisum sativum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.4 more than 90% of activity maximum at pH 7.4 and pH 8.4 Pisum sativum