Any feedback?
Literature summary for 2.7.1.4 extracted from
- Molecular and biochemical characterization of a fructose-6-phosphate-forming and ATP-dependent fructokinase of the hyperthermophilic archaeon Thermococcus litoralis (2004), Extremophiles, 8, 301-308.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
ATP | pH 7.5, 80°C | Thermococcus litoralis |  |
| 2.3 | - |
D-fructose | pH 7.5, 80°C | Thermococcus litoralis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 43.7% of activity with Mg2+ | Thermococcus litoralis | |
| Mg2+ | - |
Thermococcus litoralis | |
| Mn2+ | 65.2% of activity with Mg2+ | Thermococcus litoralis | |
| additional information | not active: Ca2+, Zn2+ | Thermococcus litoralis | |
| Ni2+ | 8.7% of activity with Mg2+ | Thermococcus litoralis | |
| Sr2+ | 1% of activity with Mg2+ | Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE, 2 * 36221, calculated for His-tagged protein | Thermococcus litoralis |
| 36221 | - |
2 * 35000, SDS-PAGE, 2 * 36221, calculated for His-tagged protein | Thermococcus litoralis |
| 58000 | - |
gel filtration | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | - |
expression in Escherichia coli with C-terminal His-tag | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 730 | - |
substrate D-fructose, pH 7.5, 80°C | Thermococcus litoralis |
| 920 | - |
substrate ATP, pH 7.5, 80°C | Thermococcus litoralis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, PBS buffer, stable for at least 3 months | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | highly specific for D-fructose, minor products are D-fructose 2-phosphate and D-fructose-3 phosphate | Thermococcus litoralis | ADP + D-fructose 6-phosphate | - |
? | |
| CTP + D-fructose | 16% of activity with ATP | Thermococcus litoralis | CDP + D-fructose 6-phosphate | - |
? | |
| GTP + D-fructose | 62% of activity with ATP | Thermococcus litoralis | GDP + D-fructose 6-phosphate | - |
? | |
| ITP + D-fructose | 73% of activity with ATP | Thermococcus litoralis | IDP + D-fructose 6-phosphate | - |
? | |
| additional information | no substrate: ADP, GDP, phosphoenolpyruvate, diphosphate | Thermococcus litoralis | ? | - |
? | |
| UTP + D-fructose | 16% of activity with ATP | Thermococcus litoralis | UDP + D-fructose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE, 2 * 36221, calculated for His-tagged protein | Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
about 1% of activity at 80°C | Thermococcus litoralis |
| 80 | - |
- |
Thermococcus litoralis |
| 100 | - |
11.7% of activity at 80°C | Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
4 h, stable | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Thermococcus litoralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Thermococcus litoralis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
