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Literature summary for 2.7.1.4 extracted from
- Cloning and characterization of two fructokinases from maize (2003), Plant Sci., 165, 1051-1058.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | above 0.5 mM; above 1 mM | Zea mays |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
ATP | pH 8.5, 30°C | Zea mays | |
| 0.109 | - |
D-fructose | pH 8.5, 30°C | Zea mays | |
| 0.159 | - |
D-fructose | pH 8.5, 30°C | Zea mays | |
| 0.187 | - |
ATP | pH 8.5, 30°C | Zea mays | |
| 0.222 | - |
UTP | pH 8.5, 30°C | Zea mays | |
| 0.491 | - |
UTP | pH 8.5, 30°C | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Zea mays | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34700 | - |
x * 34700, SDS-PAGE | Zea mays |
| 35500 | - |
x * 35500, SDS-PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | Q6XZ78 | isoform FRK2, recombinant protein with His6-tag | - |
| Zea mays | Q6XZ79 | isoform FRK1, recombinant protein with His6-tag | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 835 | - |
pH 8.5, 30°C | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Zea mays | ADP + D-fructose 6-phosphate | - |
? | |
| additional information | no substrate: glucose | Zea mays | ? | - |
? | |
| UTP + D-fructose | - |
Zea mays | UDP + D-fructose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35500, SDS-PAGE | Zea mays |
| ? | x * 34700, SDS-PAGE | Zea mays |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 494 | - |
D-fructose | pH 8.5, 30°C | Zea mays | |
| 8897 | - |
D-fructose | pH 8.5, 30°C | Zea mays | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Zea mays | calculated | - |
4.87 |
| Zea mays | calculated | - |
5.34 |
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