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Literature summary for 2.7.1.4 extracted from
- Cloning, expression, purification, crystallization and preliminary X-ray analysis of a fructokinase from Vibrio cholerae O395 (2012), Acta Crystallogr. Sect. F, 68, 1564-1567.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli BL21 as a His-taggeg fusion protein | Vibrio cholerae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Crystals of Vibrio cholerae Vc-FK and its cocrystal with fructose, ADP and Mg2+ are grown in the presence of polyethylene glycol 6000 and diffracted to 2.45 and 1.75 A resolution, respectively | Vibrio cholerae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae | A0A0H3AER7 | - |
- |
| Vibrio cholerae O395 | A0A0H3AER7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified using Ni-NTA affinity chromatography | Vibrio cholerae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Vibrio cholerae | ADP + D-fructose 6-phosphate | - |
? |  |
| ATP + D-fructose | - |
Vibrio cholerae O395 | ADP + D-fructose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cscK | - |
Vibrio cholerae |
| Vc-FK | - |
Vibrio cholerae |
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