Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | slight activation | Geobacillus stearothermophilus | |
D-fructose 1,6-diphosphate | not | Geobacillus stearothermophilus | |
D-glucose 1-phosphate | not | Geobacillus stearothermophilus | |
D-glucose 6-phosphate | activation | Geobacillus stearothermophilus | |
D-ribose 5-phosphate | activation | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | at high concentrations; substrate inhibition | Geobacillus stearothermophilus | |
Ca2+ | - |
Geobacillus stearothermophilus | |
Cu2+ | - |
Geobacillus stearothermophilus | |
Mg2+ | free form | Geobacillus stearothermophilus | |
Ni2+ | - |
Geobacillus stearothermophilus | |
phosphate | - |
Geobacillus stearothermophilus | |
Sr2+ | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not | Geobacillus stearothermophilus | |
Co2+ | can replace Mg2+ | Geobacillus stearothermophilus | |
Co2+ | requirement | Geobacillus stearothermophilus | |
K+ | requirement | Geobacillus stearothermophilus | |
Mg2+ | requirement | Geobacillus stearothermophilus | |
Mn2+ | requirement | Geobacillus stearothermophilus | |
Mn2+ | can partially replace Mg2+ | Geobacillus stearothermophilus | |
additional information | no activation by Sr2+ | Geobacillus stearothermophilus | |
additional information | no activation by Cu2+, Ni2+ | Geobacillus stearothermophilus | |
Na+ | can poorly replace K+ | Geobacillus stearothermophilus | |
NH4+ | requirement | Geobacillus stearothermophilus | |
NH4+ | can replace K+ | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Geobacillus stearothermophilus |
242000 | - |
sedimentation analysis | Geobacillus stearothermophilus |
250000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no glycoprotein | Geobacillus stearothermophilus |
Purification (Comment) | Organism |
---|---|
no isozymes | Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate = ADP + phosphoenolpyruvate | allosteric enzyme: homotropic | Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Geobacillus stearothermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
333 | - |
pH 7.2, 30°C | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-20°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years | Geobacillus stearothermophilus |
25°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days | Geobacillus stearothermophilus |
4°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years, at pH-values below 5, inactivation within a few days | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | no positive cooperativity for ADP | Geobacillus stearothermophilus | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Geobacillus stearothermophilus | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Geobacillus stearothermophilus | ATP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | poor substrate | Geobacillus stearothermophilus | CTP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Geobacillus stearothermophilus | CTP + pyruvate | - |
? | |
epsilon-ADP + phosphoenolpyruvate | poor substrate | Geobacillus stearothermophilus | ? | - |
? | |
GDP + phosphoenolpyruvate | less effective than ADP | Geobacillus stearothermophilus | GTP + pyruvate | - |
? | |
IDP + phosphoenolpyruvate | less effective than ADP | Geobacillus stearothermophilus | ITP + pyruvate | - |
? | |
additional information | broad specificity for nucleoside diphosphates | Geobacillus stearothermophilus | ? | - |
? | |
additional information | allosteric enzyme: homotropic | Geobacillus stearothermophilus | ? | - |
? | |
UDP + phosphoenolpyruvate | less effective than ADP | Geobacillus stearothermophilus | UTP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62000-64000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days | Geobacillus stearothermophilus |
65 | - |
above, rapid inactivation, in the presence of 10 mM MgCl2: t1/2: more than 30 min, 50 mM KCl protects slightly, not phosphoenolpyruvate | Geobacillus stearothermophilus |
70 | - |
5 min, inactivation, t1/2: 1 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
60°C | Geobacillus stearothermophilus |
7.2 | - |
30°C | Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.4 | 60°C, about half-maximal activity at pH 5.5 and pH 7.4 | Geobacillus stearothermophilus |
6.4 | 8.2 | 30°C, about half-maximal activity at pH 6.4 and pH 8.2 | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
below, inactivation within a few days, 4°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | allosteric effector | Geobacillus stearothermophilus | |
CMP | activation | Geobacillus stearothermophilus | |
CMP | allosteric | Geobacillus stearothermophilus | |
GDP | activation | Geobacillus stearothermophilus | |
GDP | allosteric | Geobacillus stearothermophilus | |
GMP | activation | Geobacillus stearothermophilus | |
GMP | allosteric | Geobacillus stearothermophilus |