Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | activation | Mycolicibacterium smegmatis |
General Stability | Organism |
---|---|
0.1 M KCl stabilizes enzyme in dilute solutions | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | glucose 6-phosphate reverses | Mycolicibacterium smegmatis | |
Ba2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
Ca2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
Cu2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
Ni2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
phosphate | - |
Mycolicibacterium smegmatis | |
sulfate | - |
Mycolicibacterium smegmatis | |
Zn2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Mycolicibacterium smegmatis | |
0.04 | 0.063 | phosphoenolpyruvate | - |
Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not | Mycolicibacterium smegmatis | |
Mg2+ | requirement | Mycolicibacterium smegmatis | |
Mg2+ | kinetics | Mycolicibacterium smegmatis | |
Mn2+ | requirement | Mycolicibacterium smegmatis | |
Mn2+ | can partially replace Mg2+ | Mycolicibacterium smegmatis | |
Mn2+ | kinetics | Mycolicibacterium smegmatis | |
monovalent cation | not | Mycolicibacterium smegmatis | |
additional information | no activation by Ba2+ | Mycolicibacterium smegmatis | |
additional information | no activation by Cu2+, Ni2+ | Mycolicibacterium smegmatis | |
Zn2+ | not | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230700 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis CDC 46 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate = ADP + phosphoenolpyruvate | allosteric enzyme: homotropic | Mycolicibacterium smegmatis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Mycolicibacterium smegmatis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
220 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM phosphate buffer, pH 7, 100 mM KCl, 2 mM phosphoenolpyruvate, 0.1% 2-mercaptoethanol, 50% v/v glycerol, at least 6 months | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Mycolicibacterium smegmatis | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Mycolicibacterium smegmatis | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Mycolicibacterium smegmatis CDC 46 | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Mycolicibacterium smegmatis CDC 46 | ATP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Mycolicibacterium smegmatis | CTP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Mycolicibacterium smegmatis CDC 46 | CTP + pyruvate | - |
? | |
additional information | allosteric enzyme: homotropic | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | allosteric enzyme: homotropic | Mycolicibacterium smegmatis CDC 46 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4* 57540, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Mycolicibacterium smegmatis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
up to, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M mercaptoethanol, 20% v/v glycerol, 30 min stable | Mycolicibacterium smegmatis |
70 | - |
30 min, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M 2-mercaptoethanol, 20% v/v glycerol, 85% loss of activity | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.1 | - |
Mycolicibacterium smegmatis |