Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | not | Streptococcus mutans | |
D-glucose 6-phosphate | requirement | Streptococcus mutans | |
D-ribose 5-phosphate | activation | Streptococcus mutans | |
D-Ribulose 1,5-diphosphate | not | Streptococcus mutans | |
glyceraldehyde 3-phosphate | not | Streptococcus mutans | |
additional information | no activation by glucose 1,6-diphosphate, 5'-IMP, 2',3'-AMP, 2',3'-GMP, 2',3'-UMP | Streptococcus mutans |
General Stability | Organism |
---|---|
dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7 | Streptococcus mutans |
phosphate restores activity of dialyzed, partially purified, not fully purified, enzyme preparations | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | at high concentrations; strong | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Streptococcus mutans | |
0.026 | - |
GDP | pH 7.5 | Streptococcus mutans | |
0.04 | 0.063 | phosphoenolpyruvate | pH 7.5 | Streptococcus mutans | |
0.049 | 0.1 | IDP | - |
Streptococcus mutans | |
0.22 | - |
phosphoenolpyruvate | in the presence of glucose 6-phosphate, at pH 7.5 | Streptococcus mutans | |
0.25 | 0.26 | GDP | UDP, at pH 7.5 | Streptococcus mutans | |
0.39 | - |
ADP | in the presence of glucose 6-phosphate, pH 7.5 | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not | Streptococcus mutans | |
divalent cation | requirement | Streptococcus mutans | |
divalent cation | requires both a divalent and a monovalent cation | Streptococcus mutans | |
K+ | requirement | Streptococcus mutans | |
K+ | only together with activating divalent cation | Streptococcus mutans | |
Mg2+ | requirement | Streptococcus mutans | |
Mn2+ | requirement | Streptococcus mutans | |
Mn2+ | only together with K+ | Streptococcus mutans | |
monovalent cation | requirement | Streptococcus mutans | |
monovalent cation | requires both a divalent and a monovalent cation | Streptococcus mutans | |
additional information | absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this | Streptococcus mutans | |
additional information | no activation by Li+ | Streptococcus mutans | |
Na+ | not | Streptococcus mutans | |
NH4+ | requirement | Streptococcus mutans | |
NH4+ | can replace K+ | Streptococcus mutans | |
NH4+ | only together with activating divalent cation | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Streptococcus mutans |
180000 | 190000 | gel filtration | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Streptococcus mutans JC2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus mutans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Streptococcus mutans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptococcus mutans |
Storage Stability | Organism |
---|---|
4°C, in 40 mM potassium phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 3 months | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Streptococcus mutans | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | - |
Streptococcus mutans JC2 | ATP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans | CTP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | CTP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans JC2 | CTP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | CTP + pyruvate | - |
? | |
GDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | GTP + pyruvate | - |
? | |
GDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | GTP + pyruvate | - |
? | |
IDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | ITP + pyruvate | - |
? | |
TDP + phosphoenolpyruvate | poor substrate | Streptococcus mutans | TTP + pyruvate | - |
? | |
UDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans | UTP + pyruvate | - |
? | |
UDP + phosphoenolpyruvate | less effective than ADP | Streptococcus mutans JC2 | UTP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.7 | - |
Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | not | Streptococcus mutans | |
GMP | not | Streptococcus mutans |