Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-ribose 5-phosphate | - |
Geobacillus stearothermophilus | |
additional information | not activated by fructose 1,6-bisphosphate | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain PB25 | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging and sitting drop vapour diffusion methods | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
W416F/V435W | high affinity for phosphoenolpyruvate compared to the wild-type enzyme, but its saturation curve is still sigmoidal and hyperbolic in the presence of allosteric activators | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Butyl-Toyopearl 650S chromatography and Resource Q column chromatography | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Geobacillus stearothermophilus | ATP + pyruvate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Geobacillus stearothermophilus | |
AMP | - |
Geobacillus stearothermophilus |