Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.40 extracted from

  • Turner, W.L.; Knowles, V.L.; Plaxton, W.C.
    Cytosolic pyruvate kinase: subunit composition, activity, and amount in developing castor and soybean seeds, and biochemical characterization of the purified castor seed enzyme (2005), Planta, 222, 1051-1062.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
aspartate allosteric activator Ricinus communis

General Stability

General Stability Organism
25 mM MES and 25 mM Bis-Tris propane as the assay buffer Ricinus communis
at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate 80% activity at pH 6.4, 86% activity at pH 7.4 Ricinus communis
ATP IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4 Ricinus communis
citrate IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4 Ricinus communis
D-fructose 1,6-bisphosphate IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4 Ricinus communis
diphosphate IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4 Ricinus communis
glutamate IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4 Ricinus communis
isocitrate 76% activity at pH 6.4, 91% activity at pH 7.4 Ricinus communis
malate 78% activity at pH 6.4, 90% activity at pH 7.4 Ricinus communis
additional information not inhibited by 5 mM ADP Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
ADP at pH 7.4 Ricinus communis
0.046
-
phosphoenolpyruvate at pH 7.4 Ricinus communis
0.055
-
ADP at pH 7.4, in the presence of 10 mM glutamate Ricinus communis
0.082
-
phosphoenolpyruvate at pH 6.4 Ricinus communis
0.09
-
ADP at pH 6.4 Ricinus communis
0.093
-
phosphoenolpyruvate at pH 7.4, in the presence of 10 mM glutamate Ricinus communis
0.101
-
ADP at pH 6.4, in the presence of 10 mM glutamate Ricinus communis
0.15
-
phosphoenolpyruvate at pH 6.4, in the presence of 10 mM glutamate Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-
cytosol
-
Ricinus communis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is independent of free Mg2+ concentration in the range 4-20 mM Glycine max
additional information activity is independent of free Mg2+ concentration in the range 4-20 mM Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
4 * 56000, gel filtration Glycine max
56000
-
4 * 56000, gel filtration Ricinus communis
230000
-
gel filtration Glycine max
230000
-
gel filtration Ricinus communis

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-
Ricinus communis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Butyl-Sepharose chromatography and Fractogel EMD DEAE-650 (S) anion-exchange chromatography Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Ricinus communis
-
embryo
-
Glycine max
-
endosperm developing endosperm Ricinus communis
-
seed
-
Glycine max
-
seed
-
Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.039
-
clarified extract Ricinus communis
73.1
-
after 1874fold purification Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Glycine max ATP + pyruvate
-
?
ADP + phosphoenolpyruvate
-
Ricinus communis ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 56000, gel filtration Glycine max
homotetramer 4 * 56000, gel filtration Ricinus communis

Synonyms

Synonyms Comment Organism
PKC
-
Glycine max
PKC
-
Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 7.4 displays a broad pH optimum of 6.4, however, more efficient substrate utilization is observed at pH 7.4 Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
5 8.25
-
Ricinus communis

Cofactor

Cofactor Comment Organism Structure
ADP
-
Glycine max
ADP
-
Ricinus communis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2
-
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4 Ricinus communis glutamate
8
-
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4 Ricinus communis D-fructose 1,6-bisphosphate
13.7
-
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4 Ricinus communis ATP
14.2
-
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4 Ricinus communis citrate
17.2
-
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4 Ricinus communis diphosphate