Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.40 extracted from

  • Feiden, S.; Stypa, H.; Wolfrum, U.; Wegener, G.; Kamp, G.
    A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome (2007), Reproduction, 134, 81-95.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by fructose 1,6-bisphosphate Sus scrofa
Triton X-100 50% increase of activity of the bound enzyme only Sus scrofa

General Stability

General Stability Organism
the enzyme cannot be solubilized in detergents, but trypsin without loss of activity Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by L-alanine Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02 0.05 phosphoenolpyruvate bound enzyme Sus scrofa
0.02 0.03 phosphoenolpyruvate solubilized enzyme Sus scrofa
0.46
-
ADP solubilized enzyme Sus scrofa
0.52 0.56 ADP bound enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64500
-
SDS-PAGE, subunit Sus scrofa

Organic Solvent Stability

Organic Solvent Comment Organism
additional information cannot be solubilized in detergents without loss of activity Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Fractogel EMD SO3- ion exchange chromatography and Fractogel EMD propyl (S) hydrophobic interaction chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Sus scrofa
-
erythrocyte
-
Sus scrofa
-
kidney
-
Sus scrofa
-
spermatozoon in the acrosome of the sperm head and in the fibrous sheath of the principal piece of the flagellum Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
sperm homogenate Sus scrofa
116.7
-
after 972fold purification Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Sus scrofa ATP + pyruvate
-
?

Synonyms

Synonyms Comment Organism
PK-S
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
ADP
-
Sus scrofa