Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-bisphosphate | activates isozyme PKM2 by direct binding | Homo sapiens | |
L-serine | allosteric activator of PKM2, activates isozyme PKM2 by direct binding | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H464A | site-directed mutagenesis of isozyme PKM2, the mutant shows no binding of and activation by serine | Homo sapiens |
additional information | construction of HCT116 cells silenced in both muscle isozymes PKM1 and PKM2, increased metabolic flux into the serine and glycine biosynthetic pathway in cells with reduced pyruvate kinase activity, serine and glycine deprivation decreases PKM2 activity in cells also influencing the glucose metabolism, overview | Homo sapiens |
S437Y | site-directed mutagenesis of isozyme PKM2, the mutant shows no binding of and activation by fructose 1,6-bisphosphate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the absence of extracellular serine and glycine has a pronounced inhibitory effect on pyruvate kinase activity, and serine and glycine deprivation decreases PKM2 activity in cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | Homo sapiens | - |
ADP + phosphoenolpyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
HT-29 cell | - |
Homo sapiens | - |
additional information | predominant isoform of pyruvate kinase in cancer cells is PKM2 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | - |
Homo sapiens | ADP + phosphoenolpyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PKM2 | - |
Homo sapiens |
pyruvate kinase M2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | the absence of extracellular serine and glycine has a pronounced inhibitory effect on pyruvate kinase activity | Homo sapiens |
metabolism | low pyruvate kinase activity can drive serine and glycine biosynthesis, important link between key metabolic processes observed in cancer, namely preferential PKM2 expression, aerobic glycolysis and serine biosynthesis | Homo sapiens |