Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | divalent cation required, most effective at a ratio of Mn2+ and ATP of 1:3, deviation from this ratio is inhibitory at several concentration levels | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-dehydro-3-deoxy-D-gluconate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required | Escherichia coli | |
Mg2+ | divalent cation required | Escherichia coli | |
Mn2+ | divalent cation required, most effective at a ratio of Mn2+ and ATP of 1:3, deviation from this ratio is inhibitory at several concentration levels | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella aerogenes | - |
- |
- |
no activity in Azobacter agilis | - |
- |
- |
no activity in Neurospora crassa | - |
- |
- |
no activity in Salmonella typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Escherichia coli |
Storage Stability | Organism |
---|---|
-15°C, retains more than 50% of activity after 2 weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Escherichia coli | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Klebsiella aerogenes | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
CTP + 2-dehydro-3-deoxy-D-gluconate | 8% of the activity with ATP | Escherichia coli | CDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
CTP + 2-dehydro-3-deoxy-D-gluconate | 8% of the activity with ATP | Klebsiella aerogenes | CDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
GTP + 2-dehydro-3-deoxy-D-gluconate | 34% of the activity with ATP | Escherichia coli | GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
GTP + 2-dehydro-3-deoxy-D-gluconate | 34% of the activity with ATP | Klebsiella aerogenes | GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
ITP + 2-dehydro-3-deoxy-D-gluconate | 75% of the activity with ATP | Escherichia coli | IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
ITP + 2-dehydro-3-deoxy-D-gluconate | 75% of the activity with ATP | Klebsiella aerogenes | IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
UTP + 2-dehydro-3-deoxy-D-gluconate | 7% of the activity with ATP | Escherichia coli | UDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
UTP + 2-dehydro-3-deoxy-D-gluconate | 7% of the activity with ATP | Klebsiella aerogenes | UDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6 | acetate buffer | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 8 | pH 4.7: about 50% of maximum activity, pH 8: about 85% of maximum activity, acetate buffer | Escherichia coli |