Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-dehydro-3-deoxy-D-gluconate | Thermus thermophilus | - |
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Thermus thermophilus | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxygluconate kinase | - |
Thermus thermophilus |
KDGK | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |