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Literature summary for 2.7.1.48 extracted from
- Uridine kinase from ehrlich ascites tumor: purification and properties (1960), J. Biol. Chem., 235, 3273-3279.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition of cytidine and 5-fluorouridine at substrate concentrations higher than 0.5 mM | Mus musculus | |
| p-chloromercuribenzoate | 0.17 mM, 67% inhibition, 0.69 mM, 82% inhibition, glutathione protects | Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
cytidine | pH 7.4, 37°C | Mus musculus | |
| 0.03 | - |
5-fluorouridine | pH 7.4, 37°C | Mus musculus | |
| 0.048 | - |
uridine | pH 7.4, 37°C | Mus musculus | |
| 0.5 | - |
ATP | pH 7.4, 37°C | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | can partially replace Mg2+ | Mus musculus | |
| Fe2+ | 59% of activity with Mg2+ | Mus musculus | |
| Mg2+ | required for full activity | Mus musculus | |
| Mg2+ | between 0.5/1 and 1/1 | Mus musculus | |
| Mn2+ | can partially replace Mg2+ | Mus musculus | |
| Mn2+ | 44 mM, 68% of activity with Mg2+ | Mus musculus | |
| additional information | not activated by Ca2+ and Cu2+ | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, CM-cellulose, ammonium sulfate, DEAE-cellulose, zone electrophoresis | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80.5 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 2 to 3 months, occasional thawing, no loss of activity | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-fluorocytidine | - |
Mus musculus | ADP + 5-fluorocytidine 5'-monophosphate | - |
? | |
| ATP + 5-fluorouridine | - |
Mus musculus | ADP + 5-fluorouridine 5'-monophosphate | - |
? | |
| ATP + 6-azauridine | - |
Mus musculus | ADP + 6-azauridine 5'-monophosphate | - |
? | |
| ATP + cytidine | - |
Mus musculus | ADP + CMP | - |
? | |
| ATP + uridine | no activity with CTP and UTP | Mus musculus | ADP + UMP | - |
? | |
| GTP + uridine | - |
Mus musculus | UMP + GDP | - |
? | |
| ITP + uridine | - |
Mus musculus | IDP + UMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | approx. 50% of maximal activity at pH 5.0, approx. 70% of maximal activity at pH 9.0 | Mus musculus |
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