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Literature summary for 2.7.1.48 extracted from
- Uridine-cytidine kinase from Escherichia coli (1978), Methods Enzymol., 51, 308-314.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is unstable in crude extracts, especially in the presence of Mg2+ | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | - |
Escherichia coli |  |
| UTP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
cytidine | pH 7.8, 37°C | Escherichia coli | |
| 0.35 | - |
uridine | pH 7.8, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultracentrifugation, DEAE-cellulose, affinity chromatography on 5'amino-5'-deoxyuridine | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 100 mM Tris-HCl, pH 7.8, 6 months, no loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cytidine | - |
Escherichia coli | ADP + CMP | - |
? | |
| ATP + uridine | dGTP and GTP are the most efficient phosphate donors, activity with ATP and dATP is 10% and with dUTP, dCTP and dTTP 5% of that with GTP or dGTP | Escherichia coli | ADP + UMP | - |
? | |
| dGTP + uridine | - |
Escherichia coli | dGDP + UMP | - |
? | |
| GTP + 5-fluorocytidine | 40% of activity with uridine | Escherichia coli | GDP + 5-fluorocytidine 5'-monophosphate | - |
? | |
| GTP + 5-fluorouridine | 60% of activity with uridine | Escherichia coli | GDP + 5-fluorouridine 5'-monophosphate | - |
? | |
| GTP + 6-azauridine | 31% of activity with uridine | Escherichia coli | GDP 6-azauridine 5'-monophosphate | - |
? | |
| GTP + cytidine | - |
Escherichia coli | CMP + GDP | - |
? | |
| GTP + N-acetylcytidine | 57% of activity with uridine | Escherichia coli | GDP + N-acetylcytidine 5'-monophosphate | - |
? | |
| GTP + uridine | - |
Escherichia coli | UMP + GDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
broad optimum | Escherichia coli |
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Release 2023.1 (January 2023)
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