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Literature summary for 2.7.1.49 extracted from

  • Mizote, T.; Nakayama, H.
    Purification and properties of hydroxymethylpyrimidine kinase from Escherichia coli (1989), Biochim. Biophys. Acta, 991, 109-113.
    View publication on PubMed
Search for more literature for 2.7.1.49

Inhibitors

Inhibitors Comment Organism Structure
2-methyl-4-amino-5-hydroxymethylpyrimidine competitive inhibition Escherichia coli
pyridoxine inhibits competitively the phosphorylation of 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 pyridoxine pH 6.2, 37°C Escherichia coli
0.11
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 6.2, 37°C Escherichia coli
0.24
-
 pyridoxal pH 6.2, 37°C Escherichia coli
2.4
-
 pyridoxamine pH 6.2, 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Escherichia coli 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000 44000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K-12
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.344
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + pyridoxal
-
 Escherichia coli ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Escherichia coli ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Escherichia coli ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000-44000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
EC 2.7.1.14 hydroxymethylpyrimidine kinase Escherichia coli
EC 2.7.1.35 pyridoxal kinase Escherichia coli
pyridoxal kinase
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 substrate pyridoxine Escherichia coli
6
-
 substrate 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 6 about half-maximal activity at pH 5.0 and pH 6.0, pyridoxine as substrate Escherichia coli
5.5 6.5 about half-maximal activity at pH 5.5 and pH 6.5, 2-methyl-4-amino-5-hydroxymethylpyrimidine as substrate Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0027
-
 pyridoxine pH 6.2, 37°C Escherichia coli
0.09
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 6.2, 37°C Escherichia coli