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Literature summary for 2.7.1.49 extracted from

  • Reddick, J.J.; Kinsland, C.; Nicewonger, R.; Christian, T.; Downs, D.M.; Winkler, M.E.; Begley, T.P.
    Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (1998), Tetrahedron, 54, 15983-15991.
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Application

Application Comment Organism
synthesis useful reagent for the preparation of intermediates on the thiamin biosynthetic pathway Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
pET overexpression system Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0285
-
 pyridoxine pH 8.0, 25°C Escherichia coli
0.0335
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 8.0, 25°C Escherichia coli
0.1431
-
 ATP pH 8.0, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 gel filtration indicates the enzyme is tetrameric in the native state Escherichia coli
33000
-
 4 * 33000, SDS-PAGE Escherichia coli
33534
-
 4 * 33534, predicted from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 BL21(DE3)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, stored in 10% glycerol, loses half of its activity after 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + pyridoxine
-
 Escherichia coli ADP + pyridoxine 5'-phosphate
-
 ?
additional information enzyme is probably different from previously isolated HMP kinase, because the subunit molecular masses are significantly different Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Escherichia coli
tetramer 4 * 33534, predicted from amino acid sequence Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 pyridoxine pH 8.0, 25°C Escherichia coli
0.43
-
 ATP pH 8.0, 25°C Escherichia coli
0.43
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 8.0, 25°C Escherichia coli