Cloned (Comment) | Organism |
---|---|
coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | phosphomethylpyrimidine kinase activity, EC 2.7.4.7, is abolished in C-terminally truncated isozymes Thi20p and Thi21p, the mutant yeast strains sow no thiamin phosphate synthesis activity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at 20 mM MgCl2 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | Saccharomyces cerevisiae | - |
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | Saccharomyces cerevisiae YPH500 | - |
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | Saccharomyces cerevisiae | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | Saccharomyces cerevisiae YPH500 | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21 | - |
Saccharomyces cerevisiae YPH500 | - |
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes Thi20p or Thi21p from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
thiamine synthesis from hydroxymethylpyrimidine activities in wild-type and mutant strains | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Saccharomyces cerevisiae | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Saccharomyces cerevisiae YPH500 | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | Saccharomyces cerevisiae | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | Saccharomyces cerevisiae YPH500 | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? | |
additional information | the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p | Saccharomyces cerevisiae YPH500 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
4-amino-5-hydroxymethyl-2-methylpyrimidine kinase | - |
Saccharomyces cerevisiae |
HMP kinase | - |
Saccharomyces cerevisiae |
More | the enzyme is probably identical with EC 2.7.4.7, 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase, or HMP-P kinase | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |