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Literature summary for 2.7.1.49 extracted from

  • Kawasaki, Y.; Onozuka, M.; Mizote, T.; Nosaka, K.
    Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae (2005), Curr. Genet., 47, 156-162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information phosphomethylpyrimidine kinase activity, EC 2.7.4.7, is abolished in C-terminally truncated isozymes Thi20p and Thi21p, the mutant yeast strains sow no thiamin phosphate synthesis activity Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ at 20 mM MgCl2 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Saccharomyces cerevisiae YPH500
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine Saccharomyces cerevisiae enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine Saccharomyces cerevisiae YPH500 enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-
Saccharomyces cerevisiae YPH500
-
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes Thi20p or Thi21p from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
thiamine synthesis from hydroxymethylpyrimidine activities in wild-type and mutant strains Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
Saccharomyces cerevisiae ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
Saccharomyces cerevisiae YPH500 ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase Saccharomyces cerevisiae ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase Saccharomyces cerevisiae YPH500 ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
?
additional information the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p Saccharomyces cerevisiae ?
-
?
additional information the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p Saccharomyces cerevisiae YPH500 ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
4-amino-5-hydroxymethyl-2-methylpyrimidine kinase
-
Saccharomyces cerevisiae
HMP kinase
-
Saccharomyces cerevisiae
More the enzyme is probably identical with EC 2.7.4.7, 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase, or HMP-P kinase Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae