Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Aminohydroxyethylthiazole | - |
Saccharomyces cerevisiae | |
PCMB | 0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
5-(2-hydroxyethyl)-4-methylthiazole | pH 7.5, 37°C | Saccharomyces cerevisiae | |
0.79 | - |
ATP | pH 7.5, 37°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, stimulates as effectively as Mg2+ | Saccharomyces cerevisiae | |
Mg2+ | 10 mM, stimulates as effectively as Co2+ | Saccharomyces cerevisiae | |
Mn2+ | stimulates, 42.7% as effectively as Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE | Saccharomyces cerevisiae |
470000 | - |
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole | Saccharomyces cerevisiae | the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole | ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50 | - |
Saccharomyces cerevisiae | - |
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity | - |
Purification (Comment) | Organism |
---|---|
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, about 50% loss of activity after 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 4-methyl-5-(2-hydroxyethyl)thiazole | 3.4% of the activity with ATP | Saccharomyces cerevisiae | AMP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Saccharomyces cerevisiae | ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole | the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole | Saccharomyces cerevisiae | ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
ATP + 5-(2-hydroxyethyl)-4-methylthiazole | - |
Saccharomyces cerevisiae | ADP + 4-methyl-5-(2-phosphoethyl)-thiazole | - |
? | |
CTP + 4-methyl-5-(2-hydroxyethyl)thiazole | 13.7% of the activity with ATP | Saccharomyces cerevisiae | CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
dATP + 4-methyl-5-(2-hydroxyethyl)thiazole | 24.8% of the activity with ATP | Saccharomyces cerevisiae | dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
GTP + 4-methyl-5-(2-hydroxyethyl)thiazole | 3.2% of the activity with ATP | Saccharomyces cerevisiae | GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
UTP + 4-methyl-5-(2-hydroxyethyl)thiazole | 75.1% of the activity with ATP | Saccharomyces cerevisiae | UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase | bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.2 | pH 7.4: about 80% of activity maximum, pH 9.2: about 25% of activity maximum | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
2-Aminohydroxyethylthiazole | pH 7.5, 37°C | Saccharomyces cerevisiae |