Literature summary for 2.7.1.50 extracted from

Dyguda-Kazimierowicz, E.; Sokalski, W.A.; Leszczynski, J.
Non-empirical study of the phosphorylation reaction catalyzed by 4-methyl-5-beta-hydroxyethylthiazole kinase: relevance of the theory of intermolecular interactions (2007), J. Mol. Model., 13, 839-849.

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Protein Variants

Protein Variants	Comment	Organism
C198D	increase in catalytic activity, catalytic field predictions	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	modeling of stationary points along the reaction pathway, determination of relative energies of the transition state and product	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P39593	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphooxyethyl)thiazole	SN2-like mechanism, with gamma-phosphate acting as an alcohol-activation base. Mg2+ ion 2, residues E126 and C198 play a major catalytic role, whereas Mg2+ ion 1 and C198 are responsible for product release	Bacillus subtilis	a

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Release 2023.1 (January 2023)