Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | additional Mg2+ reverses; at a Mg2+/ATP ratio below 2 | Klebsiella aerogenes | |
citrate | - |
Klebsiella aerogenes | |
D-fructose 1,6-bisphosphate | - |
Klebsiella aerogenes | |
D-fructose 6-phosphate | - |
Klebsiella aerogenes | |
GTP | at a Mg2+/GTP ratio below 2 | Klebsiella aerogenes | |
ITP | at a Mg2+/ITP ratio below 2 | Klebsiella aerogenes | |
additional information | not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | - |
Klebsiella aerogenes | |
0.75 | - |
D-Fructose 1-phosphate | - |
Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
wild-type strain PRL-R3 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months | Klebsiella aerogenes |
-20°C, crude enzyme extract prepared in water, at least 3 weeks | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | no activity with D-fructose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | no activity with D-glucose 6-phosphate, D-glucose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 1-phosphate | 35% of activity with ATP | Klebsiella aerogenes | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 1-phosphate | 43% of activity with ATP | Klebsiella aerogenes | IDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | TDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | UDP + D-fructose 1,6-bisphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.7 | approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7 | Klebsiella aerogenes |