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Literature summary for 2.7.1.56 extracted from

  • Baumann, L.; Baumann, P.
    Catabolism of D-fructose and D-ribose by Pseudomonas doudoroffii. II. Properties of 1-phosphofructokinase and 6-phosphofructokinase (1975), Arch. Microbiol., 105, 241-248.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP ATP reverses Oceanimonas doudoroffii
ATP at a Mg2+/ATP ratio below 2; free form Oceanimonas doudoroffii
K+ 300 mM, activates at 30 mM Oceanimonas doudoroffii
additional information not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by CTP and UTP; not inhibited by D-fructose 6-phosphate; not inhibited by L-glutamate; not inhibited by phosphate, AMP Oceanimonas doudoroffii
NaCl
-
Oceanimonas doudoroffii
SO42- above 25 mM Oceanimonas doudoroffii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
D-Fructose 1-phosphate
-
Oceanimonas doudoroffii
0.34
-
ATP
-
Oceanimonas doudoroffii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation at 30 mM, inhibiton at 300 mM Oceanimonas doudoroffii
Mg2+ required for activity Oceanimonas doudoroffii
Mg2+ can replace Mn2+ Oceanimonas doudoroffii
Mg2+ actual substrate: MgATP2- Oceanimonas doudoroffii
Mn2+ required for activity Oceanimonas doudoroffii
additional information not activated by Na+ Oceanimonas doudoroffii
NH4+ stimulation Oceanimonas doudoroffii

Organism

Organism UniProt Comment Textmining
Oceanimonas doudoroffii
-
wild-type strain 75
-

Purification (Commentary)

Purification (Comment) Organism
partial Oceanimonas doudoroffii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
-
Oceanimonas doudoroffii

Storage Stability

Storage Stability Organism
-15°C, 3 days, 70% loss of activity Oceanimonas doudoroffii
4°C, 1 week, about 30% loss of activity Oceanimonas doudoroffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Oceanimonas doudoroffii ADP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 1-phosphate 69% of activity with ATP Oceanimonas doudoroffii GDP + D-fructose 1,6-bisphosphate
-
?
ITP + D-fructose 1-phosphate 45% of activity with ATP Oceanimonas doudoroffii IDP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Oceanimonas doudoroffii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Oceanimonas doudoroffii

pH Range

pH Minimum pH Maximum Comment Organism
7 8 44% of maximal activity at pH 7 and 81% of maximal activity at pH 8 Oceanimonas doudoroffii