Literature summary for 2.7.1.56 extracted from

Bang, S.S.; Baumann, P.; Sawyer, M.H.
Properties of 1-phosphofructokinase from Pseudomonas putida (1977), Can. J. Microbiol., 23, 721-725.

Search for more literature for 2.7.1.56

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	no activation by pyruvate, phosphoenolpyruvate, AMP, cAMP, citrate, fructose 6-phosphate, glucose 6-phosphate, 6-phosphogluconate or 2-keto-3-deoxy-6-phosphogluconate	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	2 mM, 45% inhibition, 5 mM ATP completely relieves inhibition	Pseudomonas putida
ATP	at a Mg2+/ATP ratio below 2; free form	Pseudomonas putida
additional information	not inhibited by 6-phosphogluconate, 2-oxo-3-deoxy-6-phosphogluconate; not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by CTP and UTP; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by phosphate, AMP	Pseudomonas putida
SO42-	-	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.16	-	D-Fructose 1-phosphate	pH 8.0, 25°C	Pseudomonas putida
0.19	-	D-Fructose 1-phosphate	pH 9.0, 25°C	Pseudomonas putida
0.41	-	ATP	pH 8.0, 25°C	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	30-50 mM, 2.3fold activation	Pseudomonas putida
Mg2+	required for activity	Pseudomonas putida
Mg2+	sigmoidal response, maximal activity at 5 mM	Pseudomonas putida
Mg2+	actual substrate: MgATP2-	Pseudomonas putida
additional information	not activated by phosphate	Pseudomonas putida
Na+	30-50 mM, 1.5fold activation	Pseudomonas putida
NH4+	30-50 mM, 1.5fold activation	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-fructose 1-phosphate	Pseudomonas putida	sole pathway of D-fructose metabolism in several Pseudomonas species	ADP + D-fructose 1,6-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, DEAE-Sephadex, partially purified	Pseudomonas putida

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.02	-	-	Pseudomonas putida

Storage Stability

Storage Stability	Organism
4°C, 0.8-1.3 mg protein/ml in 50 mM phosphate buffer, pH 7, 0.5 mM dithiothreitol, 5-10% loss of activity	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 1-phosphate	-	Pseudomonas putida	ADP + D-fructose 1,6-bisphosphate	-	?
ATP + D-fructose 1-phosphate	sole pathway of D-fructose metabolism in several Pseudomonas species	Pseudomonas putida	ADP + D-fructose 1,6-bisphosphate	-	?
GTP + D-fructose 1-phosphate	-	Pseudomonas putida	GDP + D-fructose 1,6-bisphosphate	-	?
ITP + D-fructose 1-phosphate	-	Pseudomonas putida	IDP + D-fructose 1,6-bisphosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Pseudomonas putida

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10.2	very low activity at pH 5.5, approx. half-maximal activity at pH 6.3 and pH 10.2	Pseudomonas putida

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Release 2023.1 (January 2023)