Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reduced glutathione | serves as the best sulphydryl compound for optimum activity. Cysteine, 2-mercaptoethanol and dithiothreitol can substitute for glutathione contributing about 80% of the activity | Haloarcula vallismortis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Haloarcula vallismortis | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Haloarcula vallismortis | |
ADP | competitive | Haloarcula vallismortis | |
DTNB | - |
Haloarcula vallismortis | |
LiCl | - |
Haloarcula vallismortis | |
NH4Cl | activation below 0.8 mM, inhibition above 0.8 M | Haloarcula vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
D-Fructose 1-phosphate | pH 7.5, 37°C | Haloarcula vallismortis | |
1.44 | - |
ATP | pH 7.5, 37°C | Haloarcula vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CsCl | stimulates | Haloarcula vallismortis | |
KCl | stimulates, optimal concentration: 1.2 M | Haloarcula vallismortis | |
Mg2+ | is the most active divalent cation and can not be replaced either by Mn2+ or any other divalent cation. The optimal ratio of ATP/Mg2+ is 1:1 | Haloarcula vallismortis | |
NaCl | 50% of the stimulation compared to KCl | Haloarcula vallismortis | |
NH4Cl | activation below 0.8 mM, inhibition above 0.8 M | Haloarcula vallismortis | |
RbCl | stimulates | Haloarcula vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
gel filtration, sucrose density gradient ultracentrifugation | Haloarcula vallismortis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula vallismortis | - |
- |
- |
Haloarcula vallismortis ATCC 34679 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | - |
Haloarcula vallismortis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | - |
Haloarcula vallismortis ATCC 34679 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis ATCC 34679 | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis ATCC 34679 | GDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis | TDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis ATCC 34679 | TDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis | UDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 1-phosphate | besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity | Haloarcula vallismortis ATCC 34679 | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Haloarcula vallismortis |
Synonyms | Comment | Organism |
---|---|---|
1PFK | - |
Haloarcula vallismortis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloarcula vallismortis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 9.0, 0.1 M KCl, 50 mM Tris-HCl, 80% loss of activity after 2 h. The enzyme is fully active for more than 2 months in 2.5 M KCl/50 mM Tris-HCl buffer, pH 9.0. Phosphate at 50 mM concentration stabilizes the enzyme against inactivation in 0.1 M KCI for about 24 h. (NH4)2SO4 though inhibitory for expression of the enzyme activity is stabilizing | Haloarcula vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.7 | - |
steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system | Haloarcula vallismortis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.7 | steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system | Haloarcula vallismortis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
ADP | pH 7.5, 37°C | Haloarcula vallismortis |