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Literature summary for 2.7.1.56 extracted from

  • Rangaswamy, V.; Altekar, W.
    Characterization of 1-phosphofructokinase from halophilic archaebacterium Haloarcula vallismortis (1994), Biochim. Biophys. Acta, 1201, 106-112.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
reduced glutathione serves as the best sulphydryl compound for optimum activity. Cysteine, 2-mercaptoethanol and dithiothreitol can substitute for glutathione contributing about 80% of the activity Haloarcula vallismortis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
Haloarcula vallismortis
5,5'-dithiobis(2-nitrobenzoic acid)
-
Haloarcula vallismortis
ADP competitive Haloarcula vallismortis
DTNB
-
Haloarcula vallismortis
LiCl
-
Haloarcula vallismortis
NH4Cl activation below 0.8 mM, inhibition above 0.8 M Haloarcula vallismortis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
D-Fructose 1-phosphate pH 7.5, 37°C Haloarcula vallismortis
1.44
-
ATP pH 7.5, 37°C Haloarcula vallismortis

Metals/Ions

Metals/Ions Comment Organism Structure
CsCl stimulates Haloarcula vallismortis
KCl stimulates, optimal concentration: 1.2 M Haloarcula vallismortis
Mg2+ is the most active divalent cation and can not be replaced either by Mn2+ or any other divalent cation. The optimal ratio of ATP/Mg2+ is 1:1 Haloarcula vallismortis
NaCl 50% of the stimulation compared to KCl Haloarcula vallismortis
NH4Cl activation below 0.8 mM, inhibition above 0.8 M Haloarcula vallismortis
RbCl stimulates Haloarcula vallismortis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel filtration, sucrose density gradient ultracentrifugation Haloarcula vallismortis

Organism

Organism UniProt Comment Textmining
Haloarcula vallismortis
-
-
-
Haloarcula vallismortis ATCC 34679
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloarcula vallismortis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 1-phosphate
-
Haloarcula vallismortis ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate
-
Haloarcula vallismortis ATCC 34679 ADP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis CDP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 CDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis GDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 GDP + D-fructose 1,6-bisphosphate
-
?
TTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis TDP + D-fructose 1,6-bisphosphate
-
?
TTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 TDP + D-fructose 1,6-bisphosphate
-
?
UTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis UDP + D-fructose 1,6-bisphosphate
-
?
UTP + D-fructose 1-phosphate besides ATP, pyridine nucleotides such as CTP and TTP are equally effective phosphoryl donors. GTP and UTP can contribute only 60% of the activity Haloarcula vallismortis ATCC 34679 UDP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Haloarcula vallismortis

Synonyms

Synonyms Comment Organism
1PFK
-
Haloarcula vallismortis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Haloarcula vallismortis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
pH 9.0, 0.1 M KCl, 50 mM Tris-HCl, 80% loss of activity after 2 h. The enzyme is fully active for more than 2 months in 2.5 M KCl/50 mM Tris-HCl buffer, pH 9.0. Phosphate at 50 mM concentration stabilizes the enzyme against inactivation in 0.1 M KCI for about 24 h. (NH4)2SO4 though inhibitory for expression of the enzyme activity is stabilizing Haloarcula vallismortis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.7
-
steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system Haloarcula vallismortis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.7 steady increase in activity from pH 7.5 to 10.7. Activity at pHs above 10.7 are not tested due to limitations of the assay system Haloarcula vallismortis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.68
-
ADP pH 7.5, 37°C Haloarcula vallismortis