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Literature summary for 2.7.1.59 extracted from

  • Datta, A.
    Studies on hog spleen N-acetylglucosamine kinase. I. Purification and properties of N-acetylglucosamine kinase (1970), Biochim. Biophys. Acta, 220, 51-60.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2- mercaptoethanol reverses inhibition with p-chloromercuribenzoate and loss of activity during storage Sus scrofa
cysteine reverses inhibition with p-chloroxymercuribenzoate Sus scrofa

General Stability

General Stability Organism
stable to dialysis Sus scrofa
unstable to freezing and thawing, heat and low pH Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
ADP strong competitive inhibitor Sus scrofa
CTP
-
Sus scrofa
diphosphate
-
Sus scrofa
N-acetyl-D-glucosamine-6-phosphate non-competitive Sus scrofa
p-chloromercuribenzoate complete inhibition at 0.4 mM; reversible with cysteine or 2-mercaptoethanol Sus scrofa
phosphate
-
Sus scrofa
UDP-N-acetylglucosamine
-
Sus scrofa
UTP
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
N-acetyl-D-glucosamine 37°C, pH 9.0 Sus scrofa
1.8
-
ATP 37°C, pH 9.0 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Sus scrofa
Mg2+ required Sus scrofa
Mn2+ can partially replace Mg2+ in activation Sus scrofa
additional information no activity with Ca2+, Ba2+ Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Sus scrofa
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine Capra hircus
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Capra hircus
-
-
-
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-
brain
-
Capra hircus
-
kidney
-
Sus scrofa
-
kidney
-
Capra hircus
-
liver
-
Sus scrofa
-
liver
-
Capra hircus
-
muscle
-
Capra hircus
-
spleen
-
Sus scrofa
-
spleen
-
Capra hircus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.009
-
spleen, 37°C, pH 9.0 Capra hircus
0.025
-
spleen, 37°C, pH 9.0 Sus scrofa
90
-
purified enzyme, 37°C, pH 9.5 Sus scrofa

Storage Stability

Storage Stability Organism
4°C, 0.02 M potassium phosphate buffer, pH 7.6, 0.001 M EDTA, 0.01 M 2-mercaptoethanol, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-glucosamine
-
Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine
-
Capra hircus ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine highly specific Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
GTP + N-acetyl-D-glucosamine 30% of the activity with ATP Sus scrofa GDP + N-acetyl-D-glucosamine 6-phosphate
-
?
additional information D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates Sus scrofa ?
-
?
additional information no activity with UTP, CTP, ADP or phosphoenol pyruvate Sus scrofa ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
complete inactivation with 2 min Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 9.4
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.9
-
ADP 37°C, pH 9.0 Sus scrofa