Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
N-acetyl-D-glucosamine | inhibits phosphorylation of D-glucose | Rattus norvegicus | |
N-acetyl-D-mannosamine | inhibits phosphorylation of D-glucose | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
N-acetyl-D-glucosamine | enzyme from kidney, 32°C, pH 7.5 | Rattus norvegicus | |
0.06 | - |
N-acetyl-D-glucosamine | enzyme from liver, 32°C, pH 7.5 | Rattus norvegicus | |
0.1 | - |
ATP | enzyme from kidney with N-acetyl-D-glucosamine as substrate, 32°C, pH 7.5 | Rattus norvegicus | |
0.1 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.23 | - |
ATP | enzyme from liver with N-acetyl-D-glucosamine as substrate, 32°C, pH 7.5 | Rattus norvegicus | |
0.23 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.95 | - |
N-acetyl-D-mannosamine | enzyme from liver, 32°C, pH 7.5 | Rattus norvegicus | |
1 | - |
N-acetyl-D-mannosamine | enzyme from kidney, 32°C, pH 7.5 | Rattus norvegicus | |
410 | - |
D-glucose | enzyme from kidney, 32°C, pH 7.5 | Rattus norvegicus | |
600 | - |
D-glucose | enzyme from liver, 32°C, pH 7.5 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Rattus norvegicus | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from liver and kidney | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | roughly 50% of activity | Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | postulated due to kinetic data | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.5 | relatively stable at pH 6.2, rapid loss of activity above pH 8.5 | Rattus norvegicus |