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Literature summary for 2.7.1.59 extracted from
- The N-acetyl-D-glucosamine kinase of Escherichia coli and its role in murein recycling (2004), J. Bacteriol., 186, 7273-7279.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in strain XL1-Blue | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion mutants lack enzyme activity in the cytoplasm, but do not accumulate N-acetyl-D-glucosamine suggesting the existence of an alternative pathway for reutilization of N-acetyl-D-glucosamine, overview | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ZnSO4 | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
N-acetyl-D-glucosamine | pH 7.8, 37°C | Escherichia coli | |
| 0.9 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
| 37.2 | - |
D-glucose | pH 7.8, 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | Escherichia coli | enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene nagK | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 842fold from K12 strain MG1655 in a multistep procedure, recombinant enzyme from strain XL1-Blue | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0183 | - |
wild-type cell extract | Escherichia coli |
| 33.3 | - |
purified native enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified recombinant enzyme frozen in dry-ice/ethanol, fully stable for months, thawed samples retain full activity in 40% glycerol at -20°C for a few months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | 21% of the activity with N-acetyl-D-glucosamine | Escherichia coli | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | - |
Escherichia coli | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded | Escherichia coli | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| additional information | poor activity with mannose, galactose, glucosamine, D-mannosamine, N-acetylmannosamine, and N-acetylgalactosamine | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-acetyl-D-glucosamine kinase | - |
Escherichia coli |
| NagK | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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