Protein Variants | Comment | Organism |
---|---|---|
additional information | all exchanges for D37 are detrimental to the enzyme | Escherichia coli |
Y223F | activity similar to wild-type, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, less active on D-galactosamine than wild-type | Escherichia coli |
Y223H | significant reduction in activity | Escherichia coli |
Y223L | significant reduction in activity | Escherichia coli |
Y223R | significant reduction in activity | Escherichia coli |
Y223W | significant reduction in activity, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, with less efficiency than mutant Y223F, less active on D-galactosamine than wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.78 | - |
ATP | mutant Y223W | Escherichia coli | |
2.24 | - |
ATP | wild-type | Escherichia coli | |
3.58 | - |
ATP | mutant Y223F | Escherichia coli | |
4.46 | - |
D-galactose | mutant Y223F | Escherichia coli | |
5.2 | - |
D-galactose | wild-type | Escherichia coli | |
5.63 | - |
D-galactose | mutant Y223W | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
mutant Y223H | Escherichia coli |
0.63 | - |
mutant Y223R | Escherichia coli |
0.65 | - |
mutant Y223L | Escherichia coli |
0.87 | - |
mutant Y223W | Escherichia coli |
23.42 | - |
mutant Y223F | Escherichia coli |
23.5 | - |
wild-type | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-galactose | - |
Escherichia coli | ADP + 2-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
ATP + 4-deoxy-D-galactose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + 4-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
ATP + 6-deoxy-D-galactose | - |
Escherichia coli | ADP + 6-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
ATP + D-galactosamine | - |
Escherichia coli | ADP + D-galactosamine 1-phosphate | - |
? | |
ATP + D-galactose | - |
Escherichia coli | ADP + alpha-D-galactose 1-phosphate | - |
? | |
ATP + D-talose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + alpha-D-talose 1-phosphate | - |
? | |
ATP + L-glucose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + alpha-L-glucose 1-phosphate | - |
? |