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Literature summary for 2.7.1.63 extracted from
- Inorganic polyphosphate glucokinase of Mycobacterium (1964), Biochim. Biophys. Acta, 85, 283-295.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against distilled water for 10 h leads to decreased activity | Mycolicibacterium phlei |
| KCl, 0.3 M, stabilizes | Mycolicibacterium phlei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tripolyphosphate | when present in excess together with Grahams salt at pH 7.2 | Mycolicibacterium phlei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
(Phosphate)n | pH 8.5, 37°C, in terms of acid-labile phosphate | Mycolicibacterium phlei | |
| 0.28 | - |
D-glucose | pH 8.5, 37°C | Mycolicibacterium phlei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Mycolicibacterium phlei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 113000 | - |
sucrose density gradient centrifugation | Mycolicibacterium phlei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mycolicibacterium phlei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, partially purified preparation, several weeks | Mycolicibacterium phlei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)n + D-glucose | no utilization of triphosphate or tetrapolyphosphate | Mycolicibacterium phlei | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes | Mycolicibacterium phlei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Mycolicibacterium phlei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
t1/2: 24 h at 3°C or at 37°C plus 0.3 M KCl | Mycolicibacterium phlei |
| 5 | 7 | 24 h stable at 3°C | Mycolicibacterium phlei |
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Release 2023.1 (January 2023)
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