Literature summary for 2.7.1.63 extracted from

Mukai, T.; Kawai, S.; Mori, S.; Mikami, B.; Murata, K.
Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolution (2004), J. Biol. Chem., 279, 50591-50600.

Cloned(Commentary)

Cloned (Comment)	Organism
gene GMK, expression in Escherichia coli strain BL21(DE3)	Arthrobacter sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20°C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling	Arthrobacter sp.

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20°C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling

Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(phosphate)n + D-glucose	Arthrobacter sp.	-	(phosphate)n-1 + D-glucose 6-phosphate	-	?
(phosphate)n + D-glucose	Arthrobacter sp. KM	-	(phosphate)n-1 + D-glucose 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp.	-	gene GMK	-
Arthrobacter sp. KM	-	gene GMK	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli in several steps	Arthrobacter sp.

Reaction

Reaction	Comment	Organism	Reaction ID
(phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate	glucose and phosphate substrate binding site structures	Arthrobacter sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(phosphate)n + D-glucose	-	Arthrobacter sp.	(phosphate)n-1 + D-glucose 6-phosphate	-	?
(phosphate)n + D-glucose	-	Arthrobacter sp. KM	(phosphate)n-1 + D-glucose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
More	structure modeling of the C-terminal and N-terminal domains, and large and small domains, respectively	Arthrobacter sp.

Synonyms

Synonyms	Comment	Organism
GMK	-	Arthrobacter sp.
inorganic polyphosphate/ATP-glucomannokinase	-	Arthrobacter sp.
More	the enzyme belongs to the poly(P)-dependent kinase family	Arthrobacter sp.
poly(P)/ATP-glucomannokinase	-	Arthrobacter sp.