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Literature summary for 2.7.1.66 extracted from
- Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus (1972), J. Biol. Chem., 247, 5123-5131.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipid cofactor | - |
Staphylococcus aureus |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is quite stable to SDS, recovered from SDS gel slices after solubilization in 1% SDS, 1% 2-mercaptoethanol | Staphylococcus aureus |
| remarkably stable towards SDS, 8 M urea and SH-reagents | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | - |
Staphylococcus aureus |  |
| guanidine hydrochloride | strong inhibition above 100 mM | Staphylococcus aureus | |
| NaCl | above 0.5 M | Staphylococcus aureus | |
| Urea | 8 M, 20% inhibition | Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
ATP | pH 8.5, 25°C, reaction with C55-isoprenoid alcohols from Streptococcus faecalis or ficaprenol | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Staphylococcus aureus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 0.5 M, stimulates about 2-fold | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
apoprotein, gel filtration | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | - |
Staphylococcus aureus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.54 | - |
- |
Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
30 min, about 30% loss of activity | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Staphylococcus aureus |
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Release 2023.1 (January 2023)
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