Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.67 extracted from

  • Flanagan, C.A.; Thorner, J.
    Purification and characterization of a soluble phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae (1992), J. Biol. Chem., 267, 24117-24125.
    View publication on PubMed
Search for more literature for 2.7.1.67

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 stimulates Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
adenosine 10 mM, 30% inhibition; no inhibition Saccharomyces cerevisiae
ADP 5 mM, 90% inhibition Saccharomyces cerevisiae
ATPgammaS 5 mM 90% inhibition Saccharomyces cerevisiae
phosphate 20 mM, 50% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 phosphatidylinositol pH 7.5, 34°C Saccharomyces cerevisiae
0.1
-
 ATP pH 7.5, 34°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 x * 125000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Saccharomyces cerevisiae ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 125000, SDS-PAGE Saccharomyces cerevisiae