Any feedback?
Literature summary for 2.7.1.67 extracted from
- Purification, characterization, and kinetic analysis of a 55-kDa form of phosphatidylinositol 4-kinase from Saccharomyces cerevisiae (1992), J. Biol. Chem., 267, 16297-16304.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | IC50: 5.6 mM | Saccharomyces cerevisiae |  |
| HgCl2 | 5 mM, complete inhibition | Saccharomyces cerevisiae | |
| MgADP- | IC50: 0.5 mM | Saccharomyces cerevisiae | |
| NEM | 5 mM, complete inhibition | Saccharomyces cerevisiae | |
| PCMB | 5 mM, complete inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
ATP | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 0.36 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on 10 mM Mg2+ | Saccharomyces cerevisiae | |
| Mn2+ | 0.5 mM can substitute for Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | sequential reaction mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.08 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 20°C: about 65% of maximal activity, 40°C: about 65% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
unstable above | Saccharomyces cerevisiae |
| 40 | - |
t1/2: 2.1 min | Saccharomyces cerevisiae |
| 60 | - |
10 min, complete inactivation | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6.0: about 75% of maximal activity, pH 9.5: about 60% of maximal activity | Saccharomyces cerevisiae |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
IC50: 0.5 mM | Saccharomyces cerevisiae | MgADP- | |
| 5.6 | - |
IC50: 5.6 mM | Saccharomyces cerevisiae | Ca2+ | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
