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Literature summary for 2.7.1.67 extracted from
- Characterization of recombinant phosphatidylinositol 4-kinase beta reveals auto- and heterophosphorylation of the enzyme (2000), J. Biol. Chem., 275, 14642-14648.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| glutathione S-transferase PI4Kbeta fusion protein, expression in Escherichia coli | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | creation of a glutathione S-transferase PI4Kbeta fusion protein that is biologically active and phosphorylates phosphatidylinositol in ist 4-position with wortmannin sensitivity and kinetic parameters that are identical to those of purified wild-type enzyme | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme exhibits autophosphorylation that is enhanced by Mn2+ ions. Isolated C-terminal catalytic domain still displays autophosphorylation. Phosphorylation of endogenous or overexpressed PI4Kbeta is also observed in COS-7 cells | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione S-transferase PI4Kbeta fusion protein | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Bos taurus | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |  |
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Release 2023.1 (January 2023)
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