KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
ATP | isoenzyme SK2, at 1 mM shikimate | Escherichia coli | |
0.2 | - |
shikimate | isoenzyme SK2, at 5 mM ATP | Escherichia coli | |
5 | - |
shikimate | above, isoenzyme SK1, at 5 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
Co2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
Fe2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
Mg2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli | |
Mn2+ | divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
isoenzyme SK1, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Escherichia coli | SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs | ADP + shikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
two isoenzymes: SK1 and SK2 | - |
Purification (Comment) | Organism |
---|---|
isoenzyme SK1 and SK2 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | - |
isoenzyme SK2 | Escherichia coli |
410 | - |
isoenzyme SK1 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Escherichia coli | ADP + shikimate 3-phosphate | - |
? | |
ATP + shikimate | SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs | Escherichia coli | ADP + shikimate 3-phosphate | - |
? |