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Literature summary for 2.7.1.71 extracted from

  • DeFeyter, R.C.; Pittard, J.
    Purification and properties of shikimate kinase II from Escherichia coli K-12 (1986), J. Bacteriol., 165, 331-333.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
ATP
-
Escherichia coli
0.2
-
shikimate
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Co2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Fe2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Mg2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Mn2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
1 * 17000, isoenzyme SK2, SDS-PAGE Escherichia coli
21400
-
isoenzyme SK2, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
shikimate kinase II
-

Purification (Commentary)

Purification (Comment) Organism
shikimate kinase II Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
shikimate kinase II Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, protein concentration 1 mg/ml in 0.05% M Tris-HCl buffer, pH 7.5, 10 mM MgCl2, 0.1 M NaCl, 1 mM DTT, 10% loss of activity after 1 week, 90% loss of activity after 2 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
Escherichia coli ADP + shikimate 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 17000, isoenzyme SK2, SDS-PAGE Escherichia coli