Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzyme expressed in Escherichia coli | Dickeya chrysanthemi |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme K15M, sitting-drop vapor diffusion | Dickeya chrysanthemi |
Protein Variants | Comment | Organism |
---|---|---|
C13S | enzymatically active mutant, turnover-number is 65% of that of the wild-type enzyme | Dickeya chrysanthemi |
C162S | turnover-number is 1.14fold higher than that of the wild-type enzyme | Dickeya chrysanthemi |
D34N | inactive mutant enzyme | Dickeya chrysanthemi |
K15M | inactive mutant enzyme, increased thermostability and affinity for ATP when compared to the wild-type enzyme, the organization of the P-loop and flanking regions is heavily disturbed | Dickeya chrysanthemi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
shikimate | pH 7.0, 25°C, mutant enzyme C13S | Dickeya chrysanthemi | |
0.28 | - |
shikimate | pH 7.0, 25°C, mutant enzyme C162S | Dickeya chrysanthemi | |
0.67 | - |
ATP | pH 7.0, 25°C, mutant enzyme C162S | Dickeya chrysanthemi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + shikimate = ADP + 3-phosphoshikimate | the enzyme binds substrates randomly and in a synergistic fashion | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Dickeya chrysanthemi | ADP + shikimate 3-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
midpoint of protein unfolding transition is 39.7°C for the wild-type enzyme, 39°C for the mutant enzyme C13S and 43.0°C for the wild-type enzyme K15M in absence of ligands | Dickeya chrysanthemi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
ATP | pH 7.0, 25°C, mutant enzyme C13S | Dickeya chrysanthemi | |
23 | - |
shikimate | pH 7.0, 25°C, mutant enzyme C13S | Dickeya chrysanthemi | |
35 | - |
ATP | pH 7.0, 25°C, wild-type enzyme | Dickeya chrysanthemi | |
35 | - |
shikimate | pH 7.0, 25°C, wild-type enzyme | Dickeya chrysanthemi | |
40 | - |
ATP | pH 7.0, 25°C, mutant enzyme C162S | Dickeya chrysanthemi | |
40 | - |
shikimate | pH 7.0, 25°C, mutant enzyme C162S | Dickeya chrysanthemi |