Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | competitive | Streptomyces griseus | |
ADP | noncompetitive | Streptomyces griseus | |
AgNO3 | strong inhibition | Streptomyces griseus | |
AMP | noncompetitive | Streptomyces griseus | |
ATP | - |
Streptomyces griseus | |
diphosphate | noncompetitive | Streptomyces griseus | |
EDTA | strong inhibition | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
ATP | - |
Streptomyces griseus | |
3.5 | - |
streptomycin | - |
Streptomyces griseus | |
11 | - |
dihydrostreptomycin | - |
Streptomyces griseus | |
28 | - |
streptidine | - |
Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, rather less stimulatory than Mg2+ | Streptomyces griseus | |
Cu2+ | 10 mM, rather less stimulatory than Mg2+ | Streptomyces griseus | |
Fe2+ | 10 mM, slight stimulation | Streptomyces griseus | |
Mg2+ | required | Streptomyces griseus | |
Mg2+ | maximal activity with 10 mM | Streptomyces griseus | |
Mn2+ | 10 mM, slight stimulation | Streptomyces griseus | |
additional information | not affected by Ca2+, 0.2-100 mM | Streptomyces griseus | |
Zn2+ | 10 mM, slight stimulation | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, SDS-PAGE | Streptomyces griseus |
38000 | - |
gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + streptomycin | Streptomyces griseus | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | ADP + streptomycin 6-phosphate | - |
? | |
ATP + streptomycin | Streptomyces griseus HUT 6037 | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | ADP + streptomycin 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Streptomyces griseus HUT 6037 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.35 | - |
purified enzyme | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydrostreptomycin | - |
Streptomyces griseus | ADP + dihydrostreptomycin 6-phosphate | - |
? | |
ATP + dihydrostreptomycin | - |
Streptomyces griseus HUT 6037 | ADP + dihydrostreptomycin 6-phosphate | - |
? | |
ATP + streptomycin | - |
Streptomyces griseus | ADP + streptomycin 6-phosphate | - |
? | |
ATP + streptomycin | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | Streptomyces griseus | ADP + streptomycin 6-phosphate | - |
? | |
ATP + streptomycin | - |
Streptomyces griseus HUT 6037 | ADP + streptomycin 6-phosphate | - |
? | |
ATP + streptomycin | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | Streptomyces griseus HUT 6037 | ADP + streptomycin 6-phosphate | - |
? | |
additional information | no activity with GTP, CTP, UTP | Streptomyces griseus | ? | - |
? | |
additional information | no activity with GTP, CTP, UTP | Streptomyces griseus HUT 6037 | ? | - |
? | |
streptidine + ATP | - |
Streptomyces griseus | streptidine 6-phosphate + ADP | - |
? | |
streptidine + ATP | - |
Streptomyces griseus HUT 6037 | streptidine 6-phosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.6 | Streptomyces griseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces griseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
adenosine | - |
Streptomyces griseus | |
3 | - |
diphosphate | - |
Streptomyces griseus | |
5 | - |
ADP | - |
Streptomyces griseus | |
6 | - |
ATP | - |
Streptomyces griseus |