Cloned (Comment) | Organism |
---|---|
native and mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3)pLysS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C146A | very low activity | Homo sapiens |
C185A | lower efficiency with dCyd as the substrate with ATP und UTP, nearly 2-fold higher efficiency than wild-type dCK with dAdo as substrate and UTP as the phosphate donor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
UTP | wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.017 | - |
UTP | C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.019 | - |
ATP | wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.02 | - |
UTP | C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.025 | - |
ATP | C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.04 | - |
UTP | wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.087 | - |
ATP | C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 | Homo sapiens | |
0.097 | - |
ATP | wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
additional information | phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |