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Literature summary for 2.7.1.74 extracted from

  • Yu, X.C.; Miranda, M.; Liu, Z.; Patel, S.; Nguyen, N.; Carson, K.; Liu, Q.; Swaffield, J.C.
    Novel potent inhibitors of deoxycytidine kinase identified and compared by multiple assays (2010), J. Biomol. Screen., 15, 72-79.
    View publication on PubMed
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
LP-375752 i.e. 2-[4-(6-benzo[b]thiophen-2-yl-pyrimidin-4-yl)-piperazin-1-yl]-1-pyrrolidin-1-yl-ethanone Homo sapiens
LP-503392 i.e. 2-thio-2'-deoxycytidine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 2'-deoxycytidine
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinosylcytosine
-
 Homo sapiens ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
 ?
ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
ATP + cladribine
-
 Homo sapiens ADP + cladribine 5'-phosphate
-
 ?
ATP + fludarabine
-
 Homo sapiens ADP + fludarabine 5'-phosphate
-
 ?
ATP + gemcitabine
-
 Homo sapiens ADP + gemcitabine 5'-phosphate
-
 ?
additional information dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00091
-
 in 50 mM Tris (pH 7.6) Homo sapiens LP-503392
0.0138
-
 in 50 mM Tris (pH 7.6) Homo sapiens LP-375752

General Information

General Information Comment Organism
metabolism dCK plays an important role in the salvage pathway of nucleoside metabolism Homo sapiens