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Literature summary for 2.7.1.74 extracted from

  • Nagai, S.; Takenaka, K.; Nachagari, D.; Rose, C.; Domoney, K.; Sun, D.; Sparreboom, A.; Schuetz, J.D.
    Deoxycytidine kinase modulates the impact of the ABC transporter ABCG2 on clofarabine cytotoxicity (2011), Cancer Res., 71, 1781-1791.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Activating Compound

Activating Compound Comment Organism Structure
protein transporter ABCG2 the ABC transporter activates the enzyme and reduces intracellular accumulation of clofarabine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
quantitative RT-PCR enzyme expression analysis in Saos-2 cells and OCI-AML3 cells, and overexpression of wild-type and mutant enzymes in Saos2 cells, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information cotruction of a loss-of--function mutant dCK containing an altered ATP-binding site Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + clofarabine Homo sapiens
-
 ADP + clofarabine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell
-
 Homo sapiens
-
Kasumi-1 cell
-
 Homo sapiens
-
KG-1 cell
-
 Homo sapiens
-
M-07E cell
-
 Homo sapiens
-
ML-2 cell
-
 Homo sapiens
-
MV4-11 cell
-
 Homo sapiens
-
NB-4 cell
-
 Homo sapiens
-
OCI-AML3 cell
-
 Homo sapiens
-
SAOS-2 cell
-
 Homo sapiens
-
THP-1 cell
-
 Homo sapiens
-
U-937 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative enzyme activity in different cancer cell lines, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + clofarabine
-
 Homo sapiens ADP + clofarabine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function deoxycytidine kinase modulates the impact of the ABC transporter ABCG2 on clofarabine cytotoxicity in Saos-2 cells and OCI-AML3 cells, ABCG2 primarily effluxes clofarabine, but not chlorfarabine-monophosphate, overview Homo sapiens