Cloned (Comment) | Organism |
---|---|
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene yaaF, yaaF encodes an enzyme with specificity for deoxyadenosine and deoxycytidine | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 2'-deoxyadenosine | - |
Bacillus subtilis | GDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
GTP + 2'-deoxycytidine | - |
Bacillus subtilis | GDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
additional information | substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dAdo/dCyd kinase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Bacillus subtilis |