Crystallization (Comment) | Organism |
---|---|
in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site | Mycoplasma mycoides |
sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN | Mycoplasma mycoides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dATP | feedback inhibitor | Mycoplasma mycoides | |
dCTP | feedback inhibitor | Mycoplasma mycoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, Superdex 75 column gel filtration | Mycoplasma mycoides |
50000 | - |
Superdex 75 column gel filtration | Mycoplasma mycoides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma mycoides | - |
subspecies Mycoplasma mycoides mycoides | - |
Mycoplasma mycoides | Q93IG4 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 75 column gel filtration | Mycoplasma mycoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosyl cytosine | 3% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosyl cytosine | 3% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosyl uracil | 0.3% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate | - |
? | |
ATP + 2',2'-difluoro-2'-deoxycytidine | 20% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate | - |
? | |
ATP + 2',2'-difluoro-2'-deoxycytidine | 20% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2',2'-difluoro-2'-deoxyguanosine | 24% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate | - |
? | |
ATP + 2',2'-difluoro-2'-deoxyguanosine | 24% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2',3'-dideoxyadenosine | 1.5% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxyadenosine monophosphate | - |
? | |
ATP + 2',3'-dideoxyadenosine | 1.5% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxyadenosine 5'-monophosphate | - |
? | |
ATP + 2',3'-dideoxycytidine | 0.1% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxycytidine 5'-monophosphate | - |
? | |
ATP + 2',3'-dideoxyguanosine | 0.5% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxyguanosine 5'-monophosphate | - |
? | |
ATP + 2',3'-dideoxyinoosine | 3.9% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxyinosine 5'-monophosphate | - |
? | |
ATP + 2',3'-dideoxyinosine | 3.9% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2',3'-dideoxyinosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Mycoplasma mycoides | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | 81% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | 97% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyinosine | 116% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-deoxyinosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | 0.01% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyuridine | 0.9% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 2'-fluoro-arabinosyl adenine | 15% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2'-fluoro-arabinosyl adenine monophosphate | - |
? | |
ATP + 2'-fluoro-arabinosyl adenine | 15% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate | - |
? | |
ATP + 2-chloro-2'-deoxyadenosine | 111% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 2-chloro-2'-deoxyadenosine monophosphate | - |
? | |
ATP + 2-chloro-2'-deoxyadenosine | 111% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyl adenine | 77% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyl adenine | 77% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyl guanine | 6% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyl guanine | 6% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate | - |
? | |
ATP + adenosine | 7.4% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + AMP | - |
? | |
ATP + adenosine | 7.4% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + adenosine 5'-monophosphate | - |
? | |
ATP + cytidine | 0.1% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + cytidine 5'-monophosphate | - |
? | |
ATP + deoxyadenosine | 100% activity | Mycoplasma mycoides | ADP + dAMP | - |
? | |
ATP + deoxycytosine | 81% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + dCMP | - |
? | |
ATP + deoxyguanosine | 97% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + dGMP | - |
? | |
ATP + deoxyinosine | 116% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + dIMP | - |
? | |
ATP + deoxyuridine | 0.9% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + dUMP | - |
? | |
ATP + guanosine | 5.5% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + GMP | - |
? | |
ATP + guanosine | 5.5% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + guanosine 5'-monophosphate | - |
? | |
ATP + inosine | 5.9% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + IMP | - |
? | |
ATP + inosine | 5.9% of the activity with 2'-deoxyadenosine | Mycoplasma mycoides | ADP + inosine monophosphate | - |
? | |
ATP + penciclovir | 1.4% relative activity compared to deoxyadenosine | Mycoplasma mycoides | ADP + ? | - |
? | |
CTP + deoxyadenosine | 57% activity compared to ATP | Mycoplasma mycoides | CDP + dAMP | - |
? | |
dATP + deoxyadenosine | 2% activity compared to ATP | Mycoplasma mycoides | dADP + dAMP | - |
? | |
dCTP + deoxyadenosine | 106% activity compared to ATP | Mycoplasma mycoides | dCDP + dAMP | - |
? | |
dTTP + deoxyadenosine | 44% activity compared to ATP | Mycoplasma mycoides | dTDP + dAMP | - |
? | |
additional information | less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine | Mycoplasma mycoides | ? | - |
? | |
UTP + deoxyadenosine | 22% activity compared to ATP | Mycoplasma mycoides | UDP + dAMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, Superdex 75 column gel filtration | Mycoplasma mycoides |
Synonyms | Comment | Organism |
---|---|---|
dAK | - |
Mycoplasma mycoides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycoplasma mycoides |